The Effect Of Ph On Enzyme Activity

The Effect of pH on Function of Catecholase Enzyme in murphy Abstract In biochemical reactions, enzymes activity could be channelised found on these factors: parsimoniousness of subst yards, salt concentration, al loseeric regulators, temperature and pH. Inventing the hypothesis is change magnitude concentration of pH would annex the activity of enzyme Catecholase and the rate of reaction. Therefore, the purpose of this experiment was to test the frame of pH on Catecholase (one kind of enzymes in potato) activity by using pH12 buffer. By using the Spectronic 20D+ Spectrophotometer, the change in absorbance everyplace time corporation be recorded. The result proved: trend to the hypothesis, at pH 12, activity of enzyme Catecholase and the rate of reaction decreased. Introduction pH is a measure of the concentration of hydrogen H+ in source so the higher concentration hydrogen ion, the frown pH. pH is one of the most important factors affect to human body. pH was divided three parts on pH scale of measurement from pH 0 to pH 14: at pH 7, solutions are neutral. pH > 7 is alkalinity purlieu that pass on seize hydrogen ion, and pH < 7 is acidic environment that go forth donate hydrogen ion. On the other hand, increasing hydrogen ion concentration will reduce the bound surface cation concentration. change magnitude hydrogen ion concentration will increase the hydroxyl entire ion concentration (M. Chaplin, 2004). Enzymes are one of the most efficient catalysts in biochemical reactions. They are used to speed up the reaction rates by providing an alternative reaction pathway of despise activation energy, which is the minimum energy that requires for reactions occur. Catalysts take part in a reaction (chemical involved), but they do not grant any changes in chemical reaction. (Farabee, 2001) Enzymes are proteins made of a combination of different amino acids. Therefore, the enzymes may be missed thei r function when structure of protein is chan! ged under conditions such as cofactors, coenzymes,...If you heed to get a full essay, order it on our website: OrderCustomPaper.com

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